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                產(chǎn)品名稱:Human IFN-gamma

                產(chǎn)品型號(hào):SEKH-0046

                產(chǎn)品報(bào)價(jià):

                產(chǎn)品特點(diǎn):Human IFN-gamma索萊寶ELISA試劑盒的優(yōu)勢(shì):
                1,包被的酶標(biāo)板單板可拆(能拆分成12個(gè)8孔的酶標(biāo)條)
                2,提供免費(fèi)代測(cè)代檢服務(wù),代出實(shí)驗(yàn)數(shù)據(jù)
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                SEKH-0046Human IFN-gamma的詳細(xì)資料:

                Human IFN-gamma 

                中文名稱人干擾素γ檢測(cè)試劑盒
                英文名稱Human IFN-γ ELISA KIT
                ReactivityHuman
                Sample typeSerum, plasma, Cell culture supernatant
                Sensitivity4 pg/ml
                Detection TargetIFN-γ
                ApplicationsSandwich ELISA

                Human IFN-gamma

                PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is captured by the coated antibody after incubation. Following extensive washing, a biotin-conjugate antibody specific for IFN-γ is added to detect the captured IFN-γ protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by Tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm

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